Zhanelya Baiduisen

Zhanelya Baiduisen
Zhanelya Baiduisen, BSc
Research Assistant Alumni

Since I was a little kid I was fascinated with the human body and underlying biology, always questioning every little detail. What is life? Who are we? How human beings became so advanced in the process of evolution? These were the main questions that sparked my interest in a mysterious world that is biology. When choosing the career path I want to be able to answer not only “what” I do, but also “why” I do what I do. And this perfectly explains why I chose to be a scientist. I am committed to making the world a better place through collective scientific effort. 

I recently graduated with Honors in Bachelor of Science from the University of Toronto, where I specialized in Biotechnology. Currently, I am working  at Nazarbayev University in Prof. Dr. Gonzalo Hortelano’s lab. As a learner, an explorer and an aspiring scientist I want to challenge myself, and kick myself out of my comfort zone in order to maximize my knowledge in the field I am passionate about.


Education
  • University of Toronto, Honours Bachelor of Science (Biotechnology Specialist), 2016.
    • Relative Courses: Microbiology, Advance Analytical Chemistry, Biometrics, Cell and Molecular biology, Structural Biochemistry, Phylogenetic Principles, Advanced Genetic, Medical Biotechnology, Biology of Cancer
Experience
  • February, 2017–December, 2017: Research Assistant at Nazarbayev University, School of Science and Technology, Astana, Kazakhstan 
    • Tissue Culture of HT1080 cells
    • Transformation of E.coli bacteria with plasmid DNA and selection of transformants
    • Prepare DNA Maxiprep for plasmid DNA
    • Restriction digest of plasmid DNA
    • Establish PCR condition for the generation of custom proteins using PCR mutagenesis
    • Establish PCR condition for the generation of a C-terminal deletion mutants using PCR
    • Design PCR primers for cloning the coding region of genes 
    • PCR amplify open reading frame and clone into Green fluorescent protein vector (EGFP)
    • Transfect cultured cancer cells with EGFP vectors and determine the localization of the protein using fluorescent microscopy
  • December–February, 2017 Intern at SK Pharmacy, Department on work with clients, Manager, Astana, Kazakhstan
    • Maintained constant contact with existing customers, organized work with them
    • Preparation, maintenance and archiving of contracts, control of delivery of goods to the clients
    • Constants interaction with managers from Logistic and Work with suppliers departments 
    • Order of medications and constant control of requests through Portal
  • January 2015May 2015: Pharmacy Technician at I.D.A Pharmacy, Toronto, Canada
    • Maintained alphabetical storage of all medications and kept track of inspiration dates.
    • Assisted patients with good observational skills and were able to work with others as a part of team
    • Received orders from drug companies and maintained the pharmacy stocks
    • Concentrated while performing tasks and applied mathematical skills
    • Remained calm in stressful situations and efficiently fulfilled the requests in a busy pharmacy in downtown.
    • Handling cashier responsibilities when requested
Skills
  • Cell and Molecular Biology:
    • Tissue fractionation and differential centrifugation, isolate intact mitochondra and mitochondrial DNA
    • Isolation of total cellular DNA from plant tissue and DNA purification
    • Isolation of total cellular DNA from animal tissue using a kit
    • Restriction enzyme digestion, Mitochondrial enzyme activity, PCR amplification
    • Bacterial ligation and transformation, DNA Quantificatin using NanoDrop 8000
    • Extraction and electrophoresis of protein (SDS-PAGE) , Immunoblot preparation and development (Western blot)
    • Bioinformatics: Genetic Screening Using BLAST Queries and Alignment, Identifying Organism Using DNA Barcodes
    • Phylogenetic methods to find protein homologies, charactering an unknown protein using PSI-BLAST
  • Techniques in Biological Chemistry:
    • Dansylation of dipeptides and amino acids, using Thin chromatograph layer (cellulose and silica TLC) for peptide sequencing
    • Enzyme kinetic and specificity study for alkaline phosphatise that follow Michaelis-Menton kinetics.
    • Determination of Myologlobin Stability by Visible Spectroscopy
    • Purification of Glutamate-Oxaloacetate Transaminase from Pig Heart (Ion Exchange chromatography on Carboxymethyl Cellulose for proteion isolation)
  • Microbiology:
    • Bacteria morphology and staining, bacterial metabolism, medical microbiology testing, control growth
    • Transformation and DNA recombination
    • Plasmid mini-preparation of pAMP/KAN recombinants
    • Restriction enzyme analysis
    • The aims test
    • Immunology: latex agglutination slide tests
  • Analytical Chemistry Principles:
    • Fluorescence Spectroscopy, Fourier Transform Infrared Spectroscopy, Atomic Spectroscopy (Atomic Absorption and Atomic Emission), X-Ray Fluorescence and Absorption Spectroscopy, Mass Spectroscopy, Polarography and Voltammetry, Fundamentals of Separation Science: Gas Chromatography, High Performance Liquid Chromatography