Limara Manarbek

Research Assistant

  • LinkedIn

Education

1. MSc in Biological Sciences, Nazarbayev University, Nur-Sultan, Kazakhstan (2016–2018)

Department of Biological Sciences, School of Science and Technology

Graduated with Honor

Thesis title – “Novel c-Met and p14ARF crosstalk in prostate cancer progression and carbon nano dots effect on cell death”

3. BSc in Biological Sciences, Nazarbayev University, Nur-Sultan, Kazakhstan  (2011 –2016)

Department of Biological Sciences, School of Science and Technology


 

Experience

1. Research Assistant. PI: Jeannette Kunz. Nazarbayev University.

Performing and reporting on experimental laboratory studies focusing on the analysis of Sorting nexin 3 (SNX3).

2. Master Student. PI: Yingqiu Xie. Nazarbayev University.

Thesis: Novel c-Met and p14arf crosstalk in prostate cancer progression and carbon nanodots effect on cell death

3. Research Intern. PI: Yan Zhang. Institut Pasteur of Shanghai.

Learning CRISPR/Cas9 technology on HEK293T cells. Performing knock out of STUB1 gene in single cell colonies.

4. Research Intern. PI: Shangwei Hou. Shanghai Jiao Tong University.

Learning CRISPR/Cas9 technology on gastric cancer cell lines. Performing knock out of KCNH1 gene.

5. Research Intern. PI: Ainur Akilzhanova. National Laboratory Astana. 

Mapping of eco-social and genetic factors of susceptibility to tuberculosis among the population of the Republic of Kazakhstan; The relevance between breast cancer and biochemical indicators of blood in patients.

6. Summer Intern. PI: Winter Duncanson. Nazarbayev University Research and Innovation System.

Project on controlled release of alginate microparticles. Constructing microfluidic devices and working on encapsulation methods.

 

Skills

  • MICROBIOLOGY 

• Aseptic technique • Growth of bacterial culture • Liquid and agar media preparation • Plate counts    

• Spectrophotometry • Light microscope • Gram’s staining •Biochemical tests

  • MOLECULAR BIOLOGY

• Hemocytometer • Restriction enzyme digests • DNA ligation reactions • Transfection • Western Blot

• Plasmid purification • ELISA • Bacterial transformation • SDS-PAGE • gel electrophoresis • PCR • Cell culturing/passaging

  • GENOMICS

• DNA/RNA extraction from blood samples and tissue cultures • Nanodrop • Real time PCR • Allelic discrimination

• Database creation • Library preparation for NGS • Working with IonS5

  • BIOINFORMATICS

• Clustal W/O • MUSCLE • JalView • Mega6 • R statistical software • Python 3 • SQL • Tableau

  • MICROSCOPY

• Light microscope • Fluorescent microscope • Confocal microscope

 

Publications

1: Xie Y, Nurkesh AA, Ibragimova N, Zhanzak Z, Meyerbekova A, Alexeyeva Z, Yesbolatova A, Satayeva M, Mustafa A, Manarbek L, Maipas A, Altaikyzy A, Keneskhanova Z, Akbay B, Chen Z. Systematic analysis of NLMP suggests nuclear localization of RTK/MET kinases resemble cancer cell clearance. J Exp Clin Cancer Res. 2019 Jan 30;38(1):43. doi: 10.1186/s13046-018-1004-z. PubMed PMID: 30700325; PubMed Central PMCID: PMC6354337.

2: Xie Y, Fan H, Lu W, Yang Q, Nurkesh A, Yeleussizov T, Maipas A, Lu J, Manarbek L, Chen Z, Benassi E. Nuclear MET requires ARF and is inhibited by carbon nanodots through binding to phospho-tyrosine in prostate cancer. Oncogene. 2019 Apr;38(16):2967-2983. doi: 10.1038/s41388-018-0608-2. Epub 2018 Dec 19. PubMed PMID: 30568225; PubMed Central PMCID: PMC6474787.

3: Xie Y, Sun Q, Nurkesh AA, Lu J, Kauanova S, Feng J, Tursynkhan D, Yang Q, Kassymbek A, Karibayev M, Duisenova K, Fan H, Wang X, Manarbek L, Maipas A, Chen Z, Balanay MP. Dysregulation of YAP by ARF Stimulated with Tea derived Carbon Nanodots. Sci Rep. 2017 Nov 29;7(1):16577. doi: 10.1038/s41598-017-16441-y. PubMed PMID: 29185453; PubMed Central PMCID: PMC5707370.

4: Yerezhepov D, Manarbek L, Akilzhanova A. Genetic profile, metabolic syndrome and breast cancer risk in Kazakhstani Women. Experimental Biology. 2018 July. 74(1):74-87. doi:10.26577/EB-2018-1-1317

 

Conferences

  • AMERICAN ASSOCIATION FOR CANCER RESEARCH ANNUAL MEETING 2018  (Abstract co-author)

2018 - Chicago, USA • ”Natural product-derived carbon nanodots overcome drug resistance through regulation of Hippo pathway effectors”

  • 8TH INTERNATIONAL SYMPOSIUM ON MICROCHEMISTRY AND MICROSYSTEMS (Poster co-author)

2016 - Hong Kong • “Controlled release alginate microparticles”

  • ANNUAL CONFERENCE OF THE INTERNATIONAL LEADERSHIP ASSOCIATION (Poster co-author)

2015 - Barcelona, Spain • “Leadership in Kazakhstan: Exploring Ideal Leadership Characteristics”

Nazarbayev University
Block C4, Room 606
53 Kabanbay Batyr Ave.

Нур-Султан | Казахстан